The mitochondrial 12S ribosomal RNA (rRNA) gene was sequenced from 16 individuals representing 10 species of the genus Geomys. DNA sequences of Cratogeomys castanops, Thomomys bottae, T. umbrinus, Dipodomys phillipsii, and Pedetes capensis were included as outgroup taxa. Levels of 12S rRNA sequence divergence (average 3.5%, range 0.6–7.5%) suggested low levels of genetic differentiation within Geomys, with the transition:transversion ratio lower than that observed in other mammalian groups. Relative rate tests did not detect significant levels of rate heterogeneity between Geomys and outgroup taxa; however, certain ingroup taxa did show significant levels of rate heterogeneity when compared with other ingroup members. Phylogenies consistently supported previously identified relationships among taxa of Geomys.